Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic Sant Saran Bhojwani, Prem Kumar Dantu. Pages PDF. TISSUE CULTURE AND PLANT BREEDING | Clonal Forestry Plant. Tissue. Culture: Theory. and. Practice. By. S. S. BHOJWANI. and. M. K. RAZDAN. pp . Amsterdam Elsevier - Studies in plant science ; vii, p.: , English, Book, Online. Plant tissue culture: theory and practice / S.S. Bhojwani, M.K. Razdan.

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download Plant Tissue Culture: Theory and Practice, Volume 5 - 1st Edition. Print Book & E-Book. rating Write a review. Authors: S.S. Bhojwani M.K. Razdan. Edited by S.S. Bhojwani, M.K. Razdan. Volume 5, Saran Bhojwani. Pages v-vi: Download PDF Plant tissue culture: Theory and Practice, a Revised Edition. PDF Drive is your search engine for PDF files. As of today we have 78,, eBooks for you to download for free. No annoying ads, no download limits, enjoy .

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Price and David J. Please help improve this section by adding citations to reliable sources. Unsourced material may be challenged and removed. The specific differences in the regeneration potential of different organs and explants have various explanations.

The significant factors include differences in the stage of the cells in the cell cycle , the availability of or ability to transport endogenous growth regulators, and the metabolic capabilities of the cells. The most commonly used tissue explants are the meristematic ends of the plants like the stem tip, axillary bud tip and root tip.

Plant tissue culture: Theory and practice

These tissues have high rates of cell division and either concentrate or produce required growth regulating substances including auxins and cytokinins. Shoot regeneration efficiency in tissue culture is usually a quantitative trait that often varies between plant species and within a plant species among subspecies, varieties, cultivars , or ecotypes. Therefore, tissue culture regeneration can become complicated especially when many regeneration procedures have to be developed for different genotypes within the same species.

The three common pathways of plant tissue culture regeneration are propagation from preexisting meristems shoot culture or nodal culture , organogenesis and non-zygotic embryogenesis.

1st Edition

The propagation of shoots or nodal segments is usually performed in four stages for mass production of plantlets through in vitro vegetative multiplication but organogenesis is a common method of micropropagation that involves tissue regeneration of adventitious organs or axillary buds directly or indirectly from the explants.

Non-zygotic embryogenesis is a noteworthy developmental pathway that is highly comparable to that of zygotic embryos and it is an important pathway for producing somaclonal variants, developing artificial seeds, and synthesizing metabolites.

Due to the single cell origin of non-zygotic embryos, they are preferred in several regeneration systems for micropropagation, ploidy manipulation, gene transfer, and synthetic seed production. Nonetheless, tissue regeneration via organogenesis has also proved to be advantageous for studying regulatory mechanisms of plant development.

Choice of explant[ edit ] The tissue obtained from a plant to be cultured is called an explant. Explants can be taken from many different parts of a plant, including portions of shoots, leaves, stems, flowers, roots, single undifferentiated cells and from many types of mature cells provided are they still contain living cytoplasm and nuclei and are able de-differentiate and resume cell division. This has given rise to the concept of totipotentency of plant cells.

Plant Tissue Culture Books

The choice of explant material also determines if the plantlets developed via tissue culture are haploid or diploid. Also the risk of microbial contamination is increased with inappropriate explants.

The first method involving the meristems and induction of multiple shoots is the preferred method for the micropropagation industry since the risks of somaclonal variation genetic variation induced in tissue culture are minimal when compared to the other two methods.

Somatic embryogenesis is a method that has the potential to be several times higher in multiplication rates and is amenable to handling in liquid culture systems like bioreactors. Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols.

All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. White in , several volumes describing different aspects of PTC have been published.

Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals.


The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments.

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For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

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Advertisement Hide. Plant Tissue Culture:Triploid Production. The inner volume of these vessels is 1, ml, usually ml of medium is applied, and explants are inoculated.

Plant Tissue Culture Books

White, however, was able to achieve this without IAA, by using tissue of a hybrid of Nicotiana glauca and Nicotiana langsdorffii. Improvement of nutritional value of seed storage proteins.

Indeed, the Knudson C medium with some variations is still in use for orchid cultivation in vitro. Cell suspensions see Chap.

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